Chemical Composition, Antimicrobial and Antioxidant Activities of Eugenia Dysenterica DC Essential Oil

Danielle C. S. Oliveira, Telma Mary Kaneko, Maria Cláudia Marx Young, Cynthia Murakami, Inês Cordeiro, Paulo Roberto Hrihorowitsch Moreno

Abstract


Eugenia dysenterica DC is a native species from the Cerrado biome and can be found in several states of Brazil. This study evaluated the chemical composition, antimicrobial and antioxidant activities from the essential oils of a population collected in São Paulo state. Essential oils were obtained by conventional means, and their compositions were analyzed by GC-MS. Screening assays for antimicrobial activity were carried out by the microdilution method and the antioxidant potential was assessed by the DPPH scavenging method. The GC-MS analysis indicated that 52.63% of the essential oil is composed by oxygenated sesquiterpenes and the major compound is (-)-elema-1,3,11(13)-trien-12-ol (24.86%). The antimicrobial assay indicated MIC 42.1 µg/mL for S. aureus and MIC > 10000 µg/mL for the other tested microorganisms, Gram negative bacteria and fungi. The oil showed an IC50 of 5.4±0.632 mg/mL for in the DPPH assay. The essential oil had a different chemical composition from previous studies. The essential oil did not present a potent antioxidant activity. However, it can be considered a promising antimicrobial agent against S. aureus.

Keywords


Eugenia Dysenterica; Essential Oil; Antioxidant Activity; Antimicrobial Activity; GC-MS.

References


Landrum, L.R.; Kawasaki, M.L. “The genera of Myrtaceae in Brazil: an illustrated synoptic treatment and identification keys.” Brittonia 49, no.4 (1997):508–536 doi:10.2307/2807742.

Vieira, R.F.; Costa, T.S.A.; Silva, D.B.; Ferreira, F.R.; Sano, S.M. “Frutas nativas da região Centro-Oeste do Brasil, First Edition” (2006).

Ramos, M.F.S; Siani, A.C.; Souza, M.C.; Rosas, E.C.; Henriques, M. G. M. O. “Avaliação da atividade antiinflamatória dos óleos essenciais de cinco espécies de Myrtaceae”. Revista Fitos 2, no.2 (2006):58-66.

Mendonça, R.S.; Chaves, L,J.; Naves, R.V. “Caracterização de frutos e árvores de cagaita (Eugenia dysenterica DC) no sudeste do estado de Goiás, Brasil.” Revista Brasileira de Fruticultura 23, no.2 (2001):330-334. doi: 10.1590/S0100-29452001000200026.

Almeida, S.P.; Proença, C.E.B.; Sano, S.M.; Ribeiro, J.F. “Cerrado: espécies vegetais úteis, First Edition.” (1998).

Costa, T.R.; Fernandes, O.F.L.; Santos, S.C.; Oliveira, C. M. A.; Lião, L.M.; Ferri, P.H.; Paula, J.R.; Ferreira, H.D.; Sales, B.H.N.; Silva, M.R.R. “Antifungal activity of volatile constituents of Eugenia dysenterica leaf oil.” Journal of Ethnopharmacology 72, no.1-2 (2000):111–117. doi: 10.1016/S0378-8741(00)00214-2.

Galheigo, M.R.U.; Prado, L.C.S.; Mundin, A.M.M.; Gomes, D.O.; Chang, R.; Lima, A.M.C.; Canabrava, H.A.N.; Silva, L.B.B. “Antidiarrhoeic effect of Eugenia dysenterica DC (Myrtaceae) leaf essential oil” Natural Product Research 30, no. 10 (2016):1182-1185. doi: 10.1080/14786419.2015.1043633.

Lima, T.B.; Silva, O.N.; Silva, L.P.; Rocha, T.L.; Sá, M.F.G.; Franco, O.L.; Leonardecz, E. “In Vivo Effects of Cagaita (Eugenia dysenterica DC) Leaf Extracts on Diarrhea Treatment.” Evidence-Based Complementary Alternative Medicine 2011, (2011). doi:10.1155/2011/309390.

Prado, L.C.S.; Silva, D.B.; Silva, G.L.O.; Hiraki, K.R.N.; Canabrava, H.A.N.; Silva, L.B.B. “The Gastroprotective Effects of Eugenia dysenterica (Myrtaceae) Leaf Extract: The Possible Role of Condensed Tannins.” Biological & Pharmaceutical Bulletin 37, no.5 (2014):722–730. doi: 10.1248/bpb.b13-00514.

Cecílio, A.B.; Faria, D.B.; Oliveira, P.C.; Caldas, S.; Oliveira, D.A.; Sobral, M.E.G.; Maria Duarte, M.G.R.; Souza Moreira, C.P.S.; Cláudia Gontijo Silva, C.G.; Almeida, V.L. “Screening of Brazilian medicinal plants for antiviral activity against rotavirus.” Journal of Ethnopharmacology 141, no.3 (2012):975–981. doi:10.1016/j.jep.2012.03.031.

Bezerra, J.C.B.; Silva, I.A.; Ferreira, H.D.; Ferri, P.H.; Santos, S.C. “Molluscicidal activity against Biomphalaria glabrata of Brazilian Cerrado medicinal plants.” Fitoterapia 73, no.5 (2002):428–430. doi:10.1016/S0367-326X(02)00121-1.

Feitosa, C.M.; Barbosa, A.R.; Melo, C.H.S.; Freitas, R.M.; Fontes, J.E.N.; Coista, E.V.; Rashed, K.N., Júnior, J.S.C. “Antioxidant and anticholinesterase activities of the essential oil of Eugenia dysenterica DC.” African Journal of Pharmacy and Pharmacology 11, no.19 (2017):241-249. doi: 10.5897/AJPP2015.4438.

Vilela, E.C.; Carvalho, T.C.; Duarte, A.R.; Naves, R.R.; Santos, S.C.; Seraphin, J.C.; Ferri, P.H. “Spatial Structure of Eugenia dysenterica Based on Essential Oil Chemovariations and Implications for Conservation and Management of the Genetic Diversity of its Populations.” Journal of the Brazilian Chemical Society 23, no.10 (2012):1776–1782. doi:10.1590/S0103-50532012005000043.

Adams, R.P. “Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry, Fourth Edition.” (2007).

NIST (National Institute of Standards and Technology) U.S. Department of Commerce. Accessed on 14 June 2016. https://www.nist.gov/

Moreno, P.R.H.; Costa-Issa, F.I.C.; Rajca-Ferreira, A.K.; Pereira, M.A.A.; Kaneko, T.M. “Native Brazilian plants against nosocamial infections: A critical review on their potential and the antimicrobial methodology.” Current Topics in Medicinal Chemistry 13, no.24 (2013):3040–3078. doi: 10.2174/15680266113136660219.

Brand-Williams, W; Cuvelier, M.E.; Berset, C. “Use of a free radical method to evaluate antioxidant activity.” Food Science and Technology 28, no. 1 (1995):25-30. doi:10.1016/S0023-6438(95)80008-5.

Paulo, P.R.S. “Óleos essenciais de espécies de eugenia do cerrado: composições químicas sazonais, modificações químicas no β-cariofileno e avaliação da atividade acaricida.” Doctoral degree, Universidade de Brasília, Distrito Federal, Brazil, (2015).

Duarte, A.R.; Naves, R.R.; Santos, S.C; Seraphin, J.C.; Ferri, P.H. “Seasonal Influence on the Essential Oil Variability of Eugenia dysenterica.” Journal of the Brazilian Chemical Society 20, no.5 (2009):967-974. doi: 10.1590/S0103-50532009000500023

Duarte, A.R.; Naves, R.R.; Santos, S.C; Seraphin, J.C.; Ferri, P.H. “Genetic and Environmental Influence on Essential Oil Composition of Eugenia dysenterica.” Journal of the Brazilian Chemical Society 21, no.8 (2010):1459-1467. doi: 10.1590/S0103-50532010000800008.

Kim,C.H.; Lee, T.; Oh, I.; Nam, K.W.; Kim, K.H.; Oh, K.B.; Shin, J.; Mar, W. “Mast Cell Stabilizing Effect of (−)-Elema-1,3,11(13)-trien-12-ol and Thujopsene from Thujopsis dolabrata Is Mediated by Down-Regulation of Interleukin-4 Secretion in Antigen-Induced RBL-2H3 Cells” Biological and Pharmaceutical Bulletin 36, no.3 (2013):339–345. https://doi.org/10.1248/bpb.b12-00375.

Anthony, K.P.; Deolu-Sobogun, S.A.; Saleh, M.A. “Comprehensive assessment of antioxidant activity of essential oils.” Journal of Food Science 77, no.8 (2012):839–843. https://doi.org/10.1111/j.1750-3841.2012.02795.x.

Baratta, M.T.; Dorman, D.: Deans, S.G.; Figueiredo, C.; Barroso, J.G.; Ruberto, G. “Antimicrobial and antioxidant properties of some commercial essential oils.” Flavour and Fragrance Journal 13, no.4 (1998):235-244. doi: 10.1002/(SICI)1099-1026(1998070)13:4<235::AID-FFJ733>3.0.CO;2-T.

Victoria, F.N.; Lenardão, E.J.; Savegnago, L.; Perin, G.; Jacob, R.G.; Alves, D.; Silva, W.P.; Motta A.S.; Nascente, P.S. “Essential oil of the leaves of Eugenia uniflora L.: Antioxidant and antimicrobial properties.” Food and Chemical Toxicology 50, no.8 (2012):2668–2674. doi: 10.1016/j.fct.2012.05.002.

Chaieb, K.; Zmantar, T.; Ksouri, R.; Hajlaoui, H.; Mahdouani, K.; Abdelly, C.; Bakhrouf, A. “Antioxidant properties of the essential oil of Eugenia caryophyllata and its antifungal activity against a large number of clinical Candida species..” Mycoses 50, no.5 (2007):403–406. doi: 10.1111/j.1439-0507.2007.01391.x.

Mensor, L.L.; Menezes, F.S.; Leitão, G.G; Reis, A.S.; Santos, T.C.; Coube, C.S.; Leitão, S.G. “Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method.” Phytotherapy research 15, no.2 (2001):127–130. doi:10.1002/ptr.687.


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DOI: 10.28991/esj-2018-01160

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Copyright (c) 2018 Danielle C. S. Oliveira, Telma Mary Kaneko, Maria Cláudia Marx Young, Cynthia Murakami, Inês Cordeiro, Paulo Roberto Hrihorowitsch Moreno