The Effect of Zeolite/Chitosan Hybrid Matrix for Thermal-stabilization Enhancement on the Immobilization of Aspergillus fumigatus α-Amylase

Yandri Yandri; Hendri Ropingi; Tati Suhartati; John Hendri; Bambang Irawan; Sutopo Hadi

doi:10.28991/ESJ-2022-06-03-06

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Yandri Yandri
yandri.as@fmipa.unila.ac.id
Department of Chemistry, Lampung University, Lampung,, Indonesia http://orcid.org/0000-0002-5759-9013
Hendri Ropingi Department of Chemistry, Lampung University, Lampung,, Indonesia
Tati Suhartati Department of Chemistry, Lampung University, Lampung,, Indonesia
John Hendri Department of Chemistry, Lampung University, Lampung,, Indonesia
Bambang Irawan Department of Biology, Lampung University, Lampung,, Indonesia
Sutopo Hadi Department of Chemistry, Lampung University, Lampung,, Indonesia

Vol. 6 No. 3 (2022): June

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In this paper, the A. fumigatus α-amylase had been immobilized onto zeolite/chitosan hybrid to improve its thermal-stabilization for industrial needs. The methods applied enzyme production, isolation, partial purification, immobilization, and characterization. The optimum temperatures of the native and immobilized enzymes were 50 and 55ËšC, respectively. The native enzyme has KM of 3.478 ± 0.271 mg mL^-1 substrate and V_max of 2.211± 0.096 µmole mL^-1 min^-1, while the immobilized enzyme has KM value of 12.051 ± 4.949 mg mL^-1 substrate and V_max of 1.602 ± 0.576 µmole mL^-1 min^-1. The residual activity of the immobilized enzyme retained up 10.97% after fifth reuse cycles. The native enzyme has Î”G_i of 104.35 ± 1.09 kJ mole^-1 and t½ of 38.75 ± 1.53 min, while the immobilized enzyme has Î”G_i of 108.03 ± 0.05 kJ mole^-1 and t½ of 180.03 ± 3.31 min. According to the increase in half-life (t½), stability improvement of the A. fumigatusα-amylase was 4.65 times greater than the native enzyme. Thus, the zeolite/chitosan hybrid is used as a new supporting matrix for further enzyme immobilization to stabilize the enzymes.

Doi: 10.28991/ESJ-2022-06-03-06

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Yandri, Y., Ropingi, H., Suhartati, T., Hendri, J., Irawan, B., & Hadi, S. (2022). The Effect of Zeolite/Chitosan Hybrid Matrix for Thermal-stabilization Enhancement on the Immobilization of Aspergillus fumigatus α-Amylase. Emerging Science Journal, 6(3), 505–518. https://doi.org/10.28991/ESJ-2022-06-03-06

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The Effect of Zeolite/Chitosan Hybrid Matrix for Thermal-stabilization Enhancement on the Immobilization of Aspergillus fumigatus α-Amylase

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