Immobilization and Stabilization of Aspergillus Fumigatus α-Amylase by Adsorption on a Chitin

Yandri Yandri; Ezra Rheinsky Tiarsa; Tati Suhartati; Bambang Irawan; Sutopo Hadi

doi:10.28991/ESJ-2023-07-01-06

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Yandri Yandri
yandri.as@fmipa.unila.ac.id
Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Lampung, Lampung 35145,, Indonesia http://orcid.org/0000-0002-5759-9013
Ezra Rheinsky Tiarsa Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Lampung, Lampung 35145,, Indonesia
Tati Suhartati Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Lampung, Lampung 35145,, Indonesia
Bambang Irawan Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung, 35145,, Indonesia
Sutopo Hadi Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Lampung, Lampung 35145,, Indonesia

Vol. 7 No. 1 (2023): February

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In this research, immobilization of A. fumigatus α-amylase on chitin was studied with the main purpose to improve the characteristics of the enzyme. A series of experiments were carried out to study stability improvement, thermodynamic parameters, include k_i, Î”G_i, and t_½,and reusability of the immobilized enzyme. The experimental results indicate that significant thermal stability was achieved, as indicates by the ability of the enzyme to retain its relative activity above 39% after 80 min of incubation at 60^oC. Thermodynamic parameters, include k_i, Î”G_i, and t_½,indicate that the immobilized enzyme is more rigid, stable, and less flexible in the water, resulting in increased stability up to 1.5 times compared to that of the native enzyme. Furthermore, the immobilized enzyme was able to retain over 46% of its initial activity after six consecutive applications for starch hydrolysis, confirming the potential of chitin for the production of immobilized enzymes on an industrial scale.

Doi: 10.28991/ESJ-2023-07-01-06

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Yandri, Y., Tiarsa, E. R., Suhartati, T., Irawan, B., & Hadi, S. (2022). Immobilization and Stabilization of Aspergillus Fumigatus α-Amylase by Adsorption on a Chitin. Emerging Science Journal, 7(1), 77–89. https://doi.org/10.28991/ESJ-2023-07-01-06

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Immobilization and Stabilization of Aspergillus Fumigatus α-Amylase by Adsorption on a Chitin

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