Global Metabolic Changes by Bacillus Cyclic Lipopeptide Extracts on Stress Responses of Para Rubber Leaf

Paiboon Tunsagool; Pongsakorn Kruaweangmol; Anurag Sunpapao; Arnannit Kuyyogsuy; Janthima Jaresitthikunchai; Sittiruk Roytrakul; Wanwipa Vongsangnak

doi:10.28991/ESJ-2023-07-03-022

Authors

Paiboon Tunsagool
paiboon.tu@ku.ac.th
Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900,, Thailand https://orcid.org/0000-0002-4070-8500
Pongsakorn Kruaweangmol Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900,, Thailand
Anurag Sunpapao Agricultural Innovation and Management Division, Faculty of Natural Resources, Prince of Songkla University, Songkhla 90110,, Thailand
Arnannit Kuyyogsuy Nanomaterials Chemistry Research Unit, Department of Chemistry, Faculty of Science and Technology, Nakhon Si Thammarat Rajabhat University, Nakhon Si Thammarat 80280,, Thailand
Janthima Jaresitthikunchai Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani 12120,, Thailand
Sittiruk Roytrakul Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani 12120,, Thailand
Wanwipa Vongsangnak 5) Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand. 6) Omics Center for Agriculture, Bioresources, Food, and Health, Kasetsart University (OmiKU), Bangkok 10900,, Thailand

Vol. 7 No. 3 (2023): June

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Changing environmental conditions can generate abiotic stress, such as the scarcity of water and exposure to chemicals. This includes biotic stress like Phytophthora palmivora infection, which causes leaf fall disease and inhibits the growth rate of para rubber seedlings, resulting in economic loss. To prevent abiotic and biotic stresses, biocontrol agents such as cyclic lipopeptides (CLPs) from Bacillus spp. have been introduced to reduce the use of chemically synthesized fungicides and fertilizers. This study aimed to use Bacillus CLP extracts as a biological agent to stimulate the plant growth system in para rubber seedlings under stress conditions compared with the exogenous plant hormone (salicylic acid, SA). CLP extracts obtained from B. subtilis PTKU12 and exogenous SA were applied to the leaves of para rubber seedlings. The extracted metabolites from each treatment were analyzed by untargeted metabolomics for metabolite identification and metabolic networks under stress responses. In both treatments, 1,702 and 979 metabolites were detected in the positive and negative ion modes of electrospray ionization, respectively. The differential analysis revealed that the accumulation of up-regulated metabolites in the treatment of CLP extracts was higher than in the exogenous SA treatment, belonging to 56 metabolic pathways. The analysis of metabolic pathways indicated that CLP extracts employed alanine, aspartate, and glutamate metabolisms for stress responses leading to plant growth promotion. These findings revealed that the metabolic network for plant growth promotion induced by BacillusCLP extracts could be considered a protective option for para rubber plantations.

Doi: 10.28991/ESJ-2023-07-03-022

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Tunsagool, P., Kruaweangmol, P., Sunpapao, A., Kuyyogsuy, A., Jaresitthikunchai, J., Roytrakul, S., & Vongsangnak, W. (2023). Global Metabolic Changes by Bacillus Cyclic Lipopeptide Extracts on Stress Responses of Para Rubber Leaf. Emerging Science Journal, 7(3), 974–990. https://doi.org/10.28991/ESJ-2023-07-03-022

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Global Metabolic Changes by Bacillus Cyclic Lipopeptide Extracts on Stress Responses of Para Rubber Leaf

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Global Metabolic Changes by Bacillus Cyclic Lipopeptide Extracts on Stress Responses of Para Rubber Leaf

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Digital Transformation: Opportunities and Challenges for Leaders in the Emerging Countries in Response to Covid-19 Pandemic

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